- Start line
- Exchange 1 – Coconut Grove Convention Center
- Exchange 2 – University of Miami
- Exchange 3 – Tropical Park
- Exchange 4 – Tamiami Park
- Exchange 5 – Ambassador Church
- Exchange 6 – Zoo Miami
- Exchange 7 – Fall’s Shopping Center, Macy’s
- Exchange 8 – Old Cutler Presbyterian Church
- Exchange 9 – Village of Old Cutler Grass Lot
- Exchange 10 – Black Point Park and Marina
- Exchange 11 – Road shoulder on SW 107th Ave
- Exchange 12 – Homestead Speedway
- Exchange 13 – Plaza Licencido Benito Juarez Park
- Exchange 14 – Shoulder Pull-off area on Southern Glades Trail
- Exchange 15 – End of Southern Glades Canal Trail
- Exchange 16 – Key Largo Elementary School
- Exchange 17 – First State Bank/American Legion/Shellworld
- Exchange 18 – Coral Shores High School
- Exchange 19 – Picnic pull-off area
- Exchange 20 – Bridge parking area on shoulder of road
- Exchange 21 – Grass shoulder parking
- Exchange 22 – Gravel Shoulder
- Exchange 23 – Dolphin Research Center
- Exchange 24 – Marathon City Hall
- Exchange 25 – Marathon Community Park
- Exchange 26 – South end of 7 mile bridge boat ramp and road shoulder
- Exchange 27 – Capital Bank
- Exchange 28 – Right before bridge on gravel shoulder
- Exchange 29 – Shoulder parking south of bridge
- Exchange 30 – Sugarloaf Elementary School
- Exchange 31 – Shoulder parking
- Exchange 32
- Exchange 33 – Habitat Gravel Lot
- Exchange 34 – Auto Salvage Yard/Island Spice
- Exchange 35 – Path in front of Shell Station
- Finish line